A uniform benchmark for degron testing

A uniform benchmark for testing ssrA-derived degrons in the Escherichia coli ClpXP pathway.

Klimecka MM, Antosiewicz A*, Izert MA*, Szybowska PE, Twardowski PK, Delaunay C, Górna MW.

Molecules (2021) 26(19):5936. https://doi.org/10.3390/molecules26195936

Our work lays standards for assays used to compare engagement of SspB and ClpXP by a set of ssrA-derived degrons that can be used to fine-tune tools for protein stability control. We hope these optimized methods for protein production, in vitro degradation, ATPase actvity, binding estimates and monitoring in vivo stability can be useful for researchers working or targeted protein degradation.