Equipment
More detailed description of some of our equipment can be found on the CFCB website (CFCB is partially located in our laboratory).
Our group occupies 240m2 of laboratory and office space at the Laboratory for Structural and Biochemical Research (LBSBio).
The laboratory is fully equipped for protein crystallography, biophysics and biochemistry with the following items:
- Robots for pipetting solutions and crystallization drops: Mosquito LCP, Dragonfly
- Formulatrix system for automated imaging and storage of protein crystallization plates with a 1000 plates capacity and a UV scanner
- A Vitrobot for automatic vitrification and a glow-discharge unit (PELCO easiGlow, Ted Pella) for cryo-TEM grid preparation
- Three advanced FPLC (NGC Quest Bio-Rad, ÄKTA pure Cytiva) including connection with a refractometer, DLS and MALS
- Spectrophotometers and plate readers – Tecan Infinite M200 PRO with extended fluorescence, chemiluminescence and an automatic pipetting station module, Tecan Infinite F Plex for fluorescence anisotropy
- Octet R2 (Sartorius BioAnalytic) for Biolayer Interferometr
- Microscale Thermophoresis instrument (Monolith NT.115 Blue/Red from Nanotemper)
- Bio-Techne JESS for capillary electrophoresis and Western blotting
- Gradient PCR and real-time (RT-PCR) machines
- Incubator sets for mammalian cells, insect cells and bacterial and yeast culture, laminar flow chambers
- Sonicators and homogenizers, a set of centrifuges for cell fractionation
- a ChemiDoc MP station for UV, fluorescence and chemiluminescence imaging of gels and membranes
We also have access to home X-ray sources, NMR and CD spectrometers through the Structural Research Laboratory.
We have in-house access to Glacios 200kV microscope (ThermoFisher) through the Cryomicroscopy and Electron Diffraction Core Facility.
Services and products on offer:
We offer help in obtaining and interpretation of structural models of proteins, both experimental or computational. We can also aid in studies of protein-protein and protein-RNA interactions. Please contact us for further details.