New preprint and first home-made structure

Towards Targeted Protein Degradation - depletion of the essential GroEL protein in Escherichia coli using CLIPPERs.

Izert-Nowakowska MA, Klimecka MM, Antosiewicz A, Wróblewski K, Bandyra KJ, Góral TK, Kmiecik S, Serwa RA, Górna MW.

bioRxiv 2024.02.29.582761; https://doi.org/10.1101/2024.02.29.582761

A pioneering adventure by Matylda Izert - the first way to do protein knockdown in E. coli of unmodified, degronless targets. Using just simple expression from a plasmid encoding a fusion peptide - "CLIPPER" acting as BacPROTAC. Even tough GroEL went down!

Coming out with this work is also the first novel cryoEM structure obtained completely at the University of Warsaw - our Glacios microscope is functioning at its best in the hands of dr Tomasz Góral.

GroEL with bound GroTAC peptide

Wroblewski K, Izert-Nowakowska MA, Goral TK, Klimecka MM, Kmiecik S, Gorna MW.