Evaluation of Degron Motifs in Escherichia coli Using a Fluorescent Reporter.
Izert-Nowakowska MA, Szybowska PE, Klimecka MM, Górna MW.
Methods in Molecular Biology 3069:223-231. doi: 10.1007/978-1-0716-5508-5_16
Our first book published
Aug 11, 2026We are happy to present Degrons, the first book edited by our team! 
Degrons
Methods and Protocols
Editors: Górna MW, Klimecka MM, Izert-Nowakowska MA
Volume 3069 of the book series Methods in Molecular Biology
(published by Springer Nature | Humana Press, NY)
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
We are grateful to all the authors for their valuable contributions to this book, encompassing a useful set of protocols for all things degron- and degradation-related, across various experimental systems and model organisms. Thank you and congratulations!
As part of the collection, we contributed two chapters on different aspects of our work with bacterial degrons derived from ssrA and the E. coli ClpXP system:
Production of Purified Proteins Tagged with ssrA-Derived Degrons and Their Degradation by the Escherichia coli ClpXP System.
Klimecka MM, Izert-Nowakowska MA, Górna MW.
Methods in Molecular Biology 3069:215-222. doi: 10.1007/978-1-0716-5508-5_15
This last chapter is also available as a preprint, and has a popular-science summary on Gist.science: