AP2 phosphorylation study published

Phosphorylation of the AP2 µ2 subunit by p70S6 kinase facilitates clathrin-mediated endocytosis.

Tempes A, Brzozowska A, Węgierski T, Fasemire A, Olek K, Jastrzębski K, Liszewska E, Misztal K, Machnicka K, Macias M, Szybińska A, Sitkiewicz E, Malinowska A, Gozdz A, Wyszyńska A, Łasica A, Hoffmann-Młodzianowska M, Orzoł K, Miączyńska M, Górna MW, Pokrzywa W, Jaworski J, Malik AR.

Journal of Cell Science jcs.264867 (Published online 31 July 2026). doi: 10.1242/jcs.264867

The Malik and Jaworski teams have discovered regulation of AP2 via its phosphorylation on S45-µ2 by the kinase p70S6K, which plays a role in clathrin-mediated endocytosis in mammalian cells.

Ayomide Fasemire from our lab has contributed docking and molecular dynamics simulations to show that S45 is accessible to p70S6K, and that its phosphorylation indeed promotes the open conformation of the AP2 complex.

Congratulations for a successful collaboration!